Figure 5: Effects of anti-horseradish peroxidase affinity column on the presence of α-L-iduronidase containing plant complex N-glycans.

Shows western blot analysis using an antibody specific for plant complex N-glycans as described in the Supplementary Methods. α-L-Iduronidase was purified from transgenic maize seeds expressing the test-construct. Lane 1, 80-ng purified α-L-iduronidase; lane 2, 80 ng α-L-iduronidase after passing through an anti-horseradish peroxidase affinity column.