Figure 3: MiR-212/132 null mice are protected from transaortic constriction (TAC)-induced hypertrophy and heart failure.
From: The miRNA-212/132 family regulates both cardiac hypertrophy and cardiomyocyte autophagy
(a) Heart-to-body-weight ratios for 12-week-old miR-212/132 null (KO) and wild-type (WT) mice (n=5–6). (b) Cardiomyocyte cell size of neonatal miR-212/132 null and WT mice (n=5–6 isolations). (c–f) Heart-to-body-weight ratios (c), cardiomyocyte diameter (d), cardiac fibrosis (e) and lung wet weight (f) in Sham-operated WT mice and miR-212/132 null and WT mice 3 weeks after TAC surgery (n=4–7). Scale bar, 50 μm. (g–i) Echocardiographic analysis of cardiac dimensions and function in Sham-operated WT mice and miR-212/132 null and WT mice 3 weeks after TAC (n=4–11). (g) End-diastolic area, (h) end-systolic area and (i) fractional shortening. All values represent mean±s.e.m. *P<0.05; **P<0.01; ***P<0.005; #P<0.05 compared with WT TAC; ###P<0.005 compared with WT TAC; PSR, picrosirius red (collagen stain). DAPI, 4′,6-diamidino-2-phenylindole; WGA, wheat germ agglutinin.
