Figure 1: SWNTs can inhibit telomerase activity in vitro based on TRAP-G4 assay. | Nature Communications

Figure 1: SWNTs can inhibit telomerase activity in vitro based on TRAP-G4 assay.

From: Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

Figure 1

(a) G-quartet and schematic structure of the intramolecular G-quadruplex from human telomere sequence 5′-AG3 (T2AG3)3 in Na+ (left). C·C+ base pair and schematic structure of the intramolecular i-motif from human telomere sequence 5′-(C3TA2)3C3T-3′ (right), corresponding complementary strand to 5′-AG3 (T2AG3)3. (b) Scheme of inhibition of telomerase activity induced by SWNT-COOH. (c) Inhibition of telomerase activity induced by SWNT-COOH, SWNT-CH2OH, SWNT-CONHCH2CH2NH2 and MWNT-COOH in TRAP-G4 assay in vitro. The top panel showed a ladder of amplified extension products stained with 0.2% AgNO3 after gel electrophoresis; the black slope corresponds to serial fourfold dilutions of CNTs starting from 0.05 to 50 μg ml−1. IS indicated the bands of internal standard. (d) Telomerase activity was quantitated as the percent of the corresponding control sample containing no CNTs. The mean of three independent experiments with comparable results was shown. Error bars indicate±s.d.

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