Figure 5: Action of MPC088 on retinal ganglion cells.
From: Robust photoregulation of GABAA receptors by allosteric modulation with a propofol analogue
(a) Potentiation data obtained with trans- and cis-dominant MPC088; the cis-dominant preparation was obtained by pre-treating trans-dominant MPC088 with UV light for 5 min. Representative responses obtained from a single cell treated with 200 μM GABA (black), 2 μM GABA (red), 2 μM GABA co-applied with 10 μM of trans-dominant (blue) or cis-dominant (magenta) MPC088. Inset: aggregate data (mean±s.d.) for potentiation factor determined with trans- and cis-dominant MPC088 (left and right bars, respectively; n=6, P=0.003). (b) Direct activation data obtained with trans- and cis-dominant MPC088. Representative responses obtained from a cell treated with 200 μM GABA (black), 60 μM of trans-dominant (blue) and 60 μM of cis-dominant (magenta) MPC088. Inset: aggregate data (mean±s.d.) for direct activation by MPC088. Left, middle and right bars show results obtained with 30 μM trans-dominant (n=3), 60 μM trans-dominant (n=7) and 60μM cis-dominant MPC088 (n=4), respectively.
