Figure 3: Mature GCs provide input to new neurons in the DG.
From: Monosynaptic inputs to new neurons in the dentate gyrus
(a) Photomicrograph showing newborn cells (30 d.p.i.) expressing nuclear hGFP (green nucleus) only or both hGFP and cytoplasmic MCh (yellow nucleus, 'starter' cell) and mature traced GCs expressing MCh (red) only Scale bar, 25μm. (b) Overview of the horizontal section that includes the cells shown in (a), boxed. Nuclei are labelled with DAPI (blue) Scale bar, 50μm. (c) An additional example of the close association between a mature MCh+ traced cell (red) and a newborn 'starter cell' (red+green=yellow nucleus) Scale bar, 10μm. (d,e) Representative patch-clamp recordings from cells with dual labelling (hGFP++ and MCh+) and cells expressing only MCh at short intervals after virus injections Scale bar, 10μm. (d) 7 d.p.i (7d RTG+6d EnvA-ΔG-MCh+). (e) 14 d.p.i (14d RTG+6d EnvA-ΔG-MCh). Cells co-expressing hGFP and MCh (orange traces) show physiological properties of immature newborn GCs. Cells expressing MCh only (pink traces) show properties of mature GCs. Recordings were obtained from the same slice at each time point.
