Figure 4: Neurochemical characterization of cells trans-synaptically labelled with rabies virus.
From: Monosynaptic inputs to new neurons in the dentate gyrus
(a) MCh+ hilar cell immunoreactive for CR, a characteristic mossy cell marker. (b) MCh+ cells immunoreactive for GFAP, a marker for astrocytes. (c) GABA+-MCh+ cells in the DG and CA3c with differential morphology. Top inset shows a MCh-GABA+ cell with a small soma. Bottom inset shows a cell with a large soma in area CA3c. (d) Double-labelling of MCh and PV, a marker for interneurons, was observed. (e) NPY, a marker characteristic for interneurons was expressed in MCh+ cells. Inset shows co-localization. (f) MCh+ cell double-labelled with SST. Inset shows co-localization. (g) MCh+ cells in the horizontal nucleus of the diagonal band (HDB) were immunoreactive for ChAT, coronal section. (h) No double labelling between MCh+ cells and PV was observed in the medial septum (MS), coronal section. The markers are green, MCh+ cells red and DAPI labelling of nuclei blue. All scale bars, 25 μm.
