Figure 5: LEC input mediated synaptic plasticity of newborn GCs.
From: Monosynaptic inputs to new neurons in the dentate gyrus
(a,b,c) Horizontal sections showing confocal images of trans-synaptic labelling (MCh, red) in the EC at (a) 21 d.p.i. (b) 30 d.p.i. (c) 60 d.p.i. (d) Photomicrograph of the perforant pathway innervating mainly the O-ML of the DG at 90 d.p.i. Scale bar, 200 μm. (e) Representative traces of pairs of EPSCs evoked at 50 ms of inter-pulse intervals by stimulation of the O-ML and M-ML in mature control cells (n=8) and newborn GCs at 28–35 d.p.i. (n=6), 60–65 d.p.i. (n=5) and 89–95 d.p.i. (n=6), colour code matches the graph in f. (f) Comparison of the paired-pulse ratio (EPSC2/EPSC1) of EPSCs evoked at 50 ms inter-pulse intervals (left). *P<0.05, Student's t-test, mature control cells (n=8); newborn GCs: 28–35 d.p.i (n=6), 60–65 d.p.i. (n=5) and 89–95 d.p.i. (n=6). Data are means±s.e.m. Nuclei are DAPI (blue). DG, Dentate gyrus; LEC, lateral entorhinal cortex; PRh, perirhinal cortex; ML, molecular Layer; CA3, area CA3.
