Figure 4: miRNA-941 targets identification and functional analysis.

Cumulative distribution plots of log2-transformed gene expression fold-changes (LFC) for genes containing miR-941 target sites predicted by TargetScan (red) and all other expressed genes (grey) after transfection with miR-941 duplex or mock duplex in the three human cells lines: HSF (a), 293T (b) and HEK (c). Prevalence of the negative LFC measurements among predicted miR-941 targets indicates inhibitory effect of miR-941 duplex transfection, the y axis shows cumulative distribution function (CDF) of LFC distribution. Experimentally verified miR-941 target genes (pink) in hedgehog signalling pathway (d) and insulin signalling pathway (e).