Figure 2: (a) Tyrosine sulfotransferase activity of the RaxST protein. | Nature Communications

Figure 2: (a) Tyrosine sulfotransferase activity of the RaxST protein.

From: Tyrosine sulfation in a Gram-negative bacterium

Figure 2

The reaction was initiated by adding the Y-peptide (DYQVSSPIYDINYYTSE) alone, RaxST protein alone, or both the Y-peptide and 0.5 μg of RaxST protein. (b) RaxST activity is specific to tyrosine peptides. The reaction was incubated with water, Y-peptide, A-peptide (DAQVSSPIADINAATSE), Dopamine or Nitrophenol. (c) RaxST activity is specific to axY22. The reaction was initiated by adding axY22, axY22A (AENLSANFVEGDYVRTP) or water. NADPH production was measured at 340 nm using a spectrophotometer. Bars indicate the relative average value obtained for ΔA340/min±s.d. in three independent experiments. Different letters on the top of each bar indicate statistically significant differences between treatments. (one-way ANOVA analysis (Holm–Sidak test, P<0.05)).

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