Figure 7: In vivo and in vitro differentiation of iPSCs generated using AxOct4 and AxSox2.
From: Reprogramming to pluripotency is an ancient trait of vertebrate Oct4 and Pou2 proteins
(a) In vitro differentiation into cells of all three germ layers as shown by immunocytochemistry: endoderm (α-fetoprotein, AFP), mesoderm (α-smooth muscle actin, SMA) and ectoderm (β-Tubulin IIIb, TUJ1). Nuclei were stained with Hoechst (blue). Scale bars, 250 μm. (b) Microsections of hematoxylin and eosin-stained teratoma formed within 6–8 weeks of injecting nude mice with iPSCs generated using axolotl factors plus human factors. iPSCs had differentiated into tissues of all three germ layers: endoderm (respiratory epithelium (e)), mesoderm (cartilage (c), skeletal muscle (m)), and ectoderm (neural epithelium with rosettes (n)). Scale bars, 100 μm.
