Figure 3: Homomeric TRPP3 complexes on the plasma membrane of Xenopusoocytes contain three TRPP3 subunits.
From: Molecular mechanism of the assembly of an acid-sensing receptor ion channel complex
(a) TIRF image from an oocyte expressing TRPP3–EGFP. Immobile spots were selected (circles) for photobleaching analysis. Scale bar, 2 μm. (b) Time course of photobleaching of two representative spots in a, showing three EGFP bleaching steps (arrows). Green bar indicates EGFP excitation. (c) Distribution of observed and calculated bleaching steps for TRPP3–EGFP fluorescence spots. The 1–3 step distribution (green bars) is well fit by a binomial distribution (white bars) that assumes that each spot contains three EGFPs and that the probability of EGFP to be fluorescent is 70%.
