Figure 1: Enlarged coronary artery branches in Ednra KO mice. | Nature Communications

Figure 1: Enlarged coronary artery branches in Ednra KO mice.

From: Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling

Figure 1

(a–f) Haematoxylin-eosin staining of the frontal sections of WT (a–c) and Ednra KO(d–f) hearts. Boxed areas in a and d are magnified in b and e, respectively. Ednra KO hearts exhibit enlarged vessels in the septum (asterisks) instead of the normal septal branches (black arrows). Areas around the left coronary arteries in the free wall (red arrows) are also magnified in c and f, indicating no anatomical changes. (g–j) Coronary angiography by ink injection in WT (g,h) and Ednra KO (i,j) hearts. Boxed areas in g and i are magnified in h and j, respectively, indicating septal branch dilatation with an irregular and jagged contour. (k–p) Septal branch dilatation was diagnosed when its diameter was more than twice that of the proximal left coronary artery. Immunostaining of WT (k–m) and Ednra KO (n–p) septal branches for CD31 (k,n) and αSMA (l,o) with merged images (m,p). Smooth muscle layers are discontinuous in enlarged KO vessels (white arrowheads). All samples were collected at E17.5. RV, right ventricle; LV, left ventricle; scale bars, 500 (a,d,g,i), 200 (b,c,e,f), 100 (h,j) and 40 μm (k–p).

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