Figure 3: Spatial patterns of Wnt1-Cre-labelled cells. | Nature Communications

Figure 3: Spatial patterns of Wnt1-Cre-labelled cells.

From: Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling

Figure 3

Frontal sections of hearts from E17.5 Wnt1-Cre/R26R mice at proximal (a–f) and intermediate (g–l) level. Wnt1-Cre-labelled cells are detected by staining for β-galactosidase (β-gal) enzyme activity (a–c,g–i) and immunoreactivity (d–f,j–l). Boxed areas in a and g are magnified in b and c, and h and i, respectively. Wnt1-Cre-labelled cells converge on the proximal coronary arteries (b,c) and septal branches (h). Co-immunostaining for β-gal and αSMA shows their smooth muscle phenotype in the proximal coronary arteries (d–f) and septal branches (j–l). PV, pulmonary valve; RCA, right coronary artery; LCA, left coronary artery; RV, right ventricle; LV, left ventricle; SB, septal branch; scale bars, 200 (a,g) and 40 μm (d–f,j–l).

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