Figure 7: Dual labelling of both preotic and postotic NCCs. | Nature Communications

Figure 7: Dual labelling of both preotic and postotic NCCs.

From: Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling

Figure 7

(a,b) CM-DiI (red) and CFDA/DiO (green) were simultaneously injected into the neural fold at the levels of r3/4 (preotic, arrow) and somite 1/2 (postotic, arrowheads), respectively (a). At day3, each dye marks migratory cells in their corresponding PAs and the outflow tract (OFT). Migration of CM-DiI-labelled preotic crest cells preceded that of CFDA-DiO-labelled postotic cells (b). (c,d) Sections of the OFT reveal each dye troops through outflow cushions. OC, outflow cushion; PA2–4, the second to fourth PAs; scale bars, 200 μm (b).

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