Figure 8: Preotic NC ablation leads to coronary artery malformations. | Nature Communications

Figure 8: Preotic NC ablation leads to coronary artery malformations.

From: Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling

Figure 8

(a–f) Coronary angiography in sham-operated (a,c,e) and NC-ablated (b,d,f) embryos. Whereas the ablated embryos show no apparent great vessel abnormalities (b), they demonstrate superfluous coronary ostia (d) and enlarged septal branches (f). (g–l) Haematoxylin-eosin staining (g,h) with two-dimensional (i,j) and three-dimensional (k,l) reconstructions in sham-operated (g,i,k) and NC-ablated (h,j,l) embryos shows an enlarged septal branch with an irregular contour and the fistula to the right ventricle (white arrows) in the ablated embryo (j,l). One hundred and ninety horizontal sections of 10 μm each were used for the reconstruction. (m,n) Immunohistochemistry in the septal branch with antibodies to αSMA in sham-operated (m) and NC-ablated (n) embryos. The ablated embryo shows a discontinuous smooth muscle layer in the septal branch (white arrowheads). Ao, Aorta; LCA, left coronary artery; PA, pulmonary artery; SB, septal branch; scale bars, 1 mm (a,b), 200 (e,f),100 (c,d) and 40 μm (g,h,m,n), and 100 arbitrary units (a.u.; i–l).

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