Figure 2: RNA architecture and secondary structure of LCR in Drosophila Dscam.
From: An RNA architectural locus control region involved in Dscam mutually exclusive splicing
(a) Genomic organization of the D. melanogaster Dscam gene. Constitutive exons (in black boxes), alternative exons (in coloured boxes), docking sites (in blue oval) and introns (lines) are shown. Intronic elements (marked by saddle shapes) were reverse-complementary to upstream elements (marked by hearts) with stem–loop sizes as indicated (mean±s.d.). (b) The predicted hexamer architecture of Dscam pre-mRNA. Mutations introduced into six individual dsRNAs (I–VI) are indicated on the left or right mutated sequences (M1–M12). (c) Effects of RNA architecture and secondary structures were validated by disruptive mutations (M1–M12) and compensatory double mutations (M21, M34, M56, M78, M90 and M112). WT, wild-type. Data are expressed as mean±s.d. from three independent experiments.
