Figure 4: The LCR ‘subunits’.
From: An RNA architectural locus control region involved in Dscam mutually exclusive splicing
(a) The comparison of the effect on splicing between the disruptive and deletion mutations of dsRNAs. Deletion mutations had a much smaller effect on the inclusion of exon 6.43, exon 6.47 or exon 6.48 than disruptive mutations, suggesting that each dsRNA acts as enhancer ‘subunit’. Data are expressed as mean±s.d. from three independent experiments. * P<0.05 (Student’s t-test, two-tail). (b) Activation efficiency positively correlated with the number of enhancer ‘subunits’ in a variant-specific manner. (c) The LCR architectural complexity correlates with alternative exon 6 number. The number of species in each invertebrate lineage represented in the analysis is shown. WT, wild-type.
