Figure 8: Actin filaments are involved in release and uptake of MPs.

(a) Actin filaments were required for the release of MPs. H22 cells were incubated with cytochalasin D or blebbistatin. After 5 h, cells were washed and irradiated with ultraviolet B. After 24 h, MPs in the supernatants were isolated and counted by flow cytometry with polystyrene Latex beads (3 μm) as standard (left). The dots in the upper and lower box were generated from standard beads (3 μm) and MPs, respectively. The right shown was the combined reproducible data (n=4). Bars correspond to mean±s.d. *P<0.05, compared with control group. (b,c) PKH-26 (red)-stained H22 tumour cells were treated with or without different concentration of cytochalasin D or blebbistatin for 5 h, and after the drugs were washed away cells were incubated with CFSE (green)-labelled MPs. After 16 h, cells were harvested and observed under confocal microscope (b) or analysed by flow cytometry (c). Scale bar, 10 μm. Data are representative of three reproducible independent experiments. Bars correspond to mean±s.d. *P<0.01, compared with control group. In this figure, 10,000–30,000 events were collected for flow cytometric analysis.