Figure 3: HGAL gain-of-function mouse model. | Nature Communications

Figure 3: HGAL gain-of-function mouse model.

From: Germinal centre protein HGAL promotes lymphoid hyperplasia and amyloidosis via BCR-mediated Syk activation

Figure 3

(a) Schematic representation of the genomic structure of the mouse Sca1 locus and the Sca1-HGAL transgenic vector used to generate transgenic animals. (b) Identification of the transgenic mice (n=75) by Southern analysis of tail snip DNA after EcoRI digestion. Sca1-HGAL is indicated. (c) HGAL expression in Sca1-HGAL mice. BM and spleen cells of control (n=3) and Sca1-HGAL mice (n=3) were stained with anti-V5 antibody (green) and 4',6-diamidino-2-phenylindole (blue) and examined under a microscope. Data are representative of three independent experiments. Scale bar, 10 μm (d). Representative sections of ileum show increased-sized Peyer’s patches and prominent GCs stained with PAX5 and HGAL in Sca1-HGAL compared with control mice. All mouse images were photographed at comparable magnifications: X100, Peyer’s patches panels; X300, remaining panels. Human Peyer’s patches are shown for comparison (X40, Peyer’s patches panel, X80, remaining panels).

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