Figure 7: Testing the homology model.
From: The bipolar assembly domain of the mitotic motor kinesin-5
(a) EPR of double mutants. The panel shows the interaction of the spins at positions 671 and 791. The spectrum from the double mutant has a lower amplitude than the sum of the single mutants (shown in inset). If there was no interaction between the labels at positions 671 and 791, the spectrum from double labelled BASS would resemble the sum of spectra from single labelled protein. (b) Hydrodynamic analysis of truncated BASS proteins. Elution profiles of BASS, BASS631_835 and BASS631_790 from the gel filtration column (Superose6 GL10/30). Fraction numbers are given above each lane. The elution profiles are aligned for ease of comparison between the samples and the Stoke’s radii of the standard proteins are shown. Stoke’s radii of the BASS samples are given below each peak. BASS and BASS631_835 are eluted as monodisperse tetrameric peaks. BASS631_790 eluted in two peaks corresponding to dimer and tetramer species, of which the dimer species is the dominant species based on band intensity and the tetramer form is unstable.
