Table 1 Hydrodynamic analysis results and native molecular weight of full-length Kinesin-5, Bonsai, BASSΔ and BASS constructs.

From: The bipolar assembly domain of the mitotic motor kinesin-5

 

S -value

Rs (Å)

Native MW (kDa)

Subunit MW (kDa)

Subunit no.

FL

7.7±0.1

132±4

416±10

121

3.4

Bonsai

7.5±0.1

116±7

360±20

91

4.0

BASSΔ

3.9±0.1

72±2

115±5

88

1.3

BASS

3.9±0.1

92±0.4

149±5

35

4.3

  1. BASS, bipolar assembly; FL, full-length.
  2. The values are obtained from three separate and independent analyses and given as average±s.d. in the table.