Figure 1: miRNA profiling of humRNAs and breast tumour samples.

(a) Heat map diagram with two-way unsupervised hierarchical clustering of miRNAs and breast tumour samples. Each row represents an miRNA and each column represents a sample. The miRNA clustering tree is shown on the left (cluster A and B), and the sample clustering tree appears at the top (group one and two). The colour scale shown in the map illustrates the relative expression level of a miRNA across all samples: red represents an expression level above the mean; green represents expression lower than the mean. Black means median expression. Levels of 152 miRNAs passed filtering and shown from 51 frozen human breast samples (46 breast tumours and 5 normal mammary tissues). NL-normal-like, LA-luminal A, LB-luminal B, B-basal-like, H-HER2-enriched, C-claudin-low, U-undetermined, N-normal (normal breast tissue adjacent to tumours). (b–f) Kaplan–Meier plots of distant relapse-free survival (DRFS) based on the expression of miR-30 family members (30c, 30a, 30a*, 30b and 30e*). Associations of miR-30 members with DRFS were analysed with GSE22216 breast cancer data set (n=210) deposited by Buffa et al.⁶ To define low (blue) and high (red) expressers, samples were rank-ordered based on miRNA30 expression and the top 50% samples were defined as high expressers. Log rank test P-values are shown.