Figure 4: TWF1 is required for miR-30c functions.
From: MicroRNA-30c inhibits human breast tumour chemotherapy resistance by regulating TWF1 and IL-11
(a) Overexpression of TWF1 reversed the chemo response sensitized by miR-30c in MDA-MB-231 cells upon doxorubicin (Doxo) or paclitaxel (Taxol) treatments. Student’s t-test P=0.01 (Doxo) and 0.02 (Taxol), when comparing 30c/Vec with 30c/TWF1 (n=5). Error bars: s.d. (b) Knockdown of TWF1 by a transient transfection of siRNAs of TWF1 (siTWF1) mimicked the miR-30c overexpression to enhance the cytotoxicity of doxorubicin (Doxo) and paclitaxel (Taxol) in MDA-MB-231 cells. **P=0.0049, ****P=4.77E-05 (n=5) by a Student’s t-test. Error bars: s.d. (c) Relative expression levels of TWF1 upon siRNA transfections, measured by real-time PCR (BD Taqman assays). Student’s t-test P=7.31E-05 (n=3). Error bars: s.d. (d) Images of F-actin staining of MDA-MB-231 cells inhibited by siTWF1 knockdowns, compared with scrambled control (Scr) (X1,000, green for F-actin staining and blue for DAPI staining), white scale bars, 5 μm. Cells were collected 36 h after transfections.
