Figure 7: GATA3 regulation of miR-30c.
From: MicroRNA-30c inhibits human breast tumour chemotherapy resistance by regulating TWF1 and IL-11
(a) Scatter plots of GATA3 expression and miR-30c levels in UC set breast tumours (n=44, P=0.023, R=0.341; left panel) and Oxford breast tumour sets (GSE22220 and GSE22216; n=210, P=0.0005, Pearson’s correlation=0.31) (right panel). Linear regression analyses were performed between miRNA expression and GATA3 levels after log2 transformation. Plots were drawn using the scatterplot function found in the Car package ( http://cran.r-project.org). (b) Induced expression of miR-30c (Student’s t-test P=1.95E-08, n=5) by ectopic GATA3 in MDA-MB-231 breast cancer cells, measured by real-time PCR, compared with the vector control. Error bars: s.d. (c) 3 kb promoter regions of hsa-miR-30c1 and 30c2 with predicted GATA3-binding elements (E0 for 30c1, E1 and E2 for 30c2). PCR primers were designed to amplify the regions flanking E0 (P1) or both E1 and E2 (P2). (d) Left panel: the miR-30c promoter regions of 30c1 and 30c2 enriched by GATA3 antibody-mediated ChIP with GATA3-overexpressing MDA-MB-231 lysates, detected by real-time PCR using primers P1 and P2, respectively. Right panel: the miR-30c2 promoter enriched by GATA3 antibody-mediated ChIP with MCF-7, detected by real-time PCR with P2 primers. Student’s t-test P=0.0004 (30c1) and 3.59E-06 (30c2) for MDA-MB-231 cells and 5.23E-06 (30c2) for MCF-7 cells, respectively (n=6). Error bars: s.d.
