Figure 3: Apoptotic cell death occurs in Dullard mutant nephrons. | Nature Communications

Figure 3: Apoptotic cell death occurs in Dullard mutant nephrons.

From: The phosphatase Dullard negatively regulates BMP signalling and is essential for nephron maintenance after birth

Figure 3

(a) Immunostaining for PHH3 and Ki-67 in the 14-day-old cortex and outer medulla. Scale bar, 100 μm. (b) The numbers of PHH3- or Ki-67-positive proliferating cells do not differ between the control and mutant kidneys (n=3 in each group). Mean±s.d., NS, not significant. (c) Cleaved caspase-3-positive apoptotic cells are increased in the 14-day-old mutant cortex and outer medulla, but not in the inner medulla. Scale bar, 100 μm. (d) Cleaved caspase-3-positive cells overlap with YFP-positive mesenchyme-derived cells (green), but not with DBA-positive ureteric bud-derived cells (purple), in 7-day-old Six2Cre; Dullardflox/flox; R26RYFP mutant kidneys. Scale bar, 100 μm.

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