Figure 2: NA-induced Src activation is mediated by an ADRB/cAMP/PKA mechanism.
From: Src activation by β-adrenoreceptors is a key switch for tumour metastasis
(a) Effect of 10 μM NA on SrcY419 phosphorylation in HeyA8 and SKOV3ip1 cells. *P<0.01, **P<0.001; two-tail Student’s t-test. (b) HeyA8 cells were incubated for 1 h with 1 μM butoxamine (Buto, β2 antagonist), exposed to 10 μM NA and probed for pSrcY419. *P<0.001; two-tail Student’s t-test. (c) Similar experiments were performed with siRNA targeted against ADRB1 (ADRB1 si) or ADRB2 (ADRB2 si) in SKOV3ip1 cells. *P<0.001; two-tail Student’s t-test. (d) Effect of 50 μM dbcAMP (PKA agonist) on pSrcY419 in SKOV3ip1 cells. *P<0.01, **P<0.001; two-tail Student’s t-test. (e) HeyA8 cells were exposed to 10 μM KT5720 (PKA antagonist) for 1 h, stimulated with 10 μM NA and probed for pSrcY419. *P<0.001; two-tail Student’s t-test. (f) SKOV3ip1 cells were treated with 10 μM NA, and Src was visualized by immunofluorescence (scale bar, 9.375 μm). (g) Quantification of cellular response to NA (measured as cells with increased Src expression at the focal adhesions) is shown in graphs *P<001; two-tail Student’s t-test. In panels (a–e), the immunoblot is shown at the top and quantification of pSrc band intensity relative to the intensity of the total Src band is shown below.
