Figure 3: NA-induced SrcS17 phosphorylation on SYF cells is required for Src activation.
From: Src activation by β-adrenoreceptors is a key switch for tumour metastasis
(a) HeyA8 cells were treated with 10 μM NA and probed for pSrcS17. *P<0.01; **P<0.001; two-tail Student’s t-test. SYF-null cells transfected with either WT or mutant Src (S17A) were stimulated with 10 μM NA and immunoblotted for (b) pSrcS17 or (c) pSrcY419. *P<0.01; **P<0.001; two-tail Student’s t-test. (d) SYF-null cells transfected with either WT or mutant Src (S17A) were stimulated with 10 μM NA or PDGF (20 ng ml−1) and subjected to a migration assay or western blot analysis for pSrcS17 expression. *P<0.01; two-tail Student’s t-test. In panels (a–c), the immunoblot is shown at the top and quantification of pSrc band intensity relative to the intensity of the total Src band is shown below.
