Figure 7: Expression levels of Hsp70 inducers in skeletal muscle.

(a) Immunoblotting for Nfya, Sp1, p53 and Tbp in wild-type, AR-97Q and heterozygous Hsf-1-knockout AR-97Q mice (13 weeks old). (b,c) Quantitative analysis using densitometry revealed that the expression levels of Nfya and Sp1 in skeletal muscle were upregulated in heterozygous Hsf-1-knockout AR-97Q mice compared with AR-97Q mice (13 weeks old). (d,e) Immunoblotting for Nfya and Sp1 in the spinal cord and liver in the mice of each group (13 weeks old). (f) Immunohistochemistry of skeletal muscle in wild-type, AR-97Q and Hsf-1-knockout AR-97Q mice using anti-Nfya and anti-Sp1 antibodies (13 weeks old). The nuclear uptake of Nfya and Sp1 was upregulated in AR-97Q mice compared with wild-type mice, and further intensified in heterozygous Hsf-1-knockout AR-97Q mice. (g,h) Quantification of immunohistochemistry with Nfya and Sp1 in the skeletal muscle of heterozygous Hsf-1-knockout AR-97Q mice compared with AR-97Q mice (13 weeks old). (i) Immunoblotting for AR in wild-type, AR-97Q and Hsf-1-knockout AR-97Q mice (13 weeks old). *Nonspecific bands. Quantitative analysis of immunoblots using densitometry indicated that the expression levels of abnormal AR aggregations in the skeletal muscle had no significant change in heterozygous Hsf-1-knockout AR-97Q mice compared with AR-97Q mice (j). Unpaired t-test (n=3). Error bars, s.e.m. (b,c,g,h,j). Scale bars, 50 μm (f). NS, not significant.