Figure 8: HSF-1 alleviates neuronal atrophy in the motor cortex and striatum of AR-97Q mice. | Nature Communications

Figure 8: HSF-1 alleviates neuronal atrophy in the motor cortex and striatum of AR-97Q mice.

From: Heat shock factor-1 influences pathological lesion distribution of polyglutamine-induced neurodegeneration

Figure 8

(a,f) Expression of transgenes in the motor cortex (a) and striatum (f) of the AR-97Q mice 3 weeks after injection. (b,g) Immunohistochemistry for Hsf-1/HSF-1, 1C2 and NeuN in the motor cortex and striatum of AR-97Q mice. The regions injected with lentiviral vector expressing hHSF-1 were compared with the contralateral side of the same mouse (13 weeks old). (c–e, h–j) Quantitative analyses of the frequency of 1C2-positive cells (c,h), relative expression level of Hsf-1/HSF-1 (d,i) and cell size of neuronal cells (e,j) confirmed the neuroprotection by the lentiviral delivery of HSF-1 into the motor cortex and striatum of AR-97Q mice. Unpaired t-test. More than 300 neurons from three brains were analysed in each group (c–e,h–j). Error bars indicate s.e.m. (c–e,h–j). Scale bars, 100 μm (a,f) and 50 μm (b,g).

Back to article page