Figure 1: TM84 is a tight-binding inhibitor of the LeuRSAt aminoacylation reaction. | Nature Communications

Figure 1: TM84 is a tight-binding inhibitor of the LeuRSAt aminoacylation reaction.

From: Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase

Figure 1

(a) Aminoacylation reaction of LeuRS. (b) Comparison of Leu-AMP and TM84—differences in red. (c) Inhibition of LeuRSAt (4 nM) aminoacylation reaction +/− TM84 (3 nM). Error bars on the initial rate time points represent standard deviation, n=3. (d) The TM84 dose–response curve of LeuRSAt (4 nM) aminoacylation reaction was fit to the Morrison equation for tight-binding inhibitors (equation (1)), indicating a Ki app of 0.3±0.1 nM.

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