Figure 3: tRNA induces TM84 tight-binding and adopts the modified conformation.
From: Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase
(a) ITC titration of TM84 into LeuRSAt. The top panel shows raw thermogram and bottom panel shows the binding isotherm fitted to a single-site model (see Table 1 for thermodynamic values). (b) Titration of TM84 into LeuRSAt·tRNALeu complex. Bottom panel isotherm fitted to a two-independent-sites model. (c) Fluorescent tRNApyrLeu probe sensitive to the environment of the CCA 3′-end. Pyrrolo-Cyt base (red) located at nucleotide Cyt75. (d) Fluorescence emission spectra for tRNApyrLeu (tRNA*) free or bound to different LeuRSAt complexes.
