Table 1 Isothermal titration calorimetry data showing binding of TM84 and/or tRNALeu to LeuRSAt.
From: Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase
Complex | Model | K d (nM) | Stoichiometry | Δ H (kcal mol−1) | −TΔS (kcal mol−1) | Δ G (kcal mol−1) |
|---|---|---|---|---|---|---|
LeuRSAt·TM84 | One site | 152±20 | 0.93±0.08 | −10.4±0.3 | 0.95±0.4 | −9.4±0.08 |
LeuRSAt·tRNALeu ·TM84 | Two site | 0.81±0.31 14.4±1.37 | 0.51±0.03 0.60±0.01 | −18.8±0.9 −27.8±0.7 | 6.2±0.5 16.3±0.5 | −12.6±0.2 −10.8±0.01 |
LeuRSAt·tRNALeu | One site | 84.9±6.1 | 0.93±0.01 | −16.2±0.6 | 6.4±0.6 | −9.7±0.04 |
