Table 2 Data collection and refinement statistics.
From: Plant tumour biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase
Data collection | E. coli LeuRS+ E. coli tRNALeu(UAA)+TM84 |
|---|---|
Space group | C2 |
Cell dimensions | |
a, b, c (Å) | 158.58, 68.19, 226.22 |
α, β, γ (°) | 90°, 105.53, 90° |
Solvent content (%) | 53.5 |
Beamline | ESRF ID14-4 |
Wavelength (Å) | 0.9395 |
Detector | ADSC quantum Q315r |
Resolution range of data (last shell) (Å) | 43.59–2.4 (2.40–2.50) |
Completeness (last shell) (%) | 93.3 (65.1) |
R-sym (last shell) | 0.104 (0.435) |
I/σI (last shell) | 10.2 (1.8) |
Redundancy (last shell) | 3.91 (1.7) |
Refinement | |
No. of reflections used in refinement work (free) | 80,975 (4,280) |
R-factor (last shell) | 0.187 (0.343) |
R-free (last shell) | 0.238 (0.389) |
No.of non-H atoms: total | 17,522 |
Protein | 13,717 |
tRNA | 3,367 |
Ligand | 62 |
Solvent | 369 water, 7 Mg2+ |
Mean B-value (Å2) | 48.8 |
Quality | |
Ramachandran plot (%) | |
Favoured regions | 96.7 |
Allowed regions | 99.8 |
Disallowed regions | 0.2 (4 outliers/1,716 residues) |
R.m.s. deviations | |
Bond distances (Å) | 0.010 |
Bond angles (°) | 1.45 |
