Figure 3: Tuning translation initiation rate to improve fatty acid production.
From: Modular optimization of multi-gene pathways for fatty acids production in E. coli
(a) Selected ribosome binding sites (RBSs) are from the MIT Registry of Standard Biological Parts. Underscored sequence is RBS core sequence; Italic red sequence shows XbaI site; start codon is shown in green colour. (b) The dose response of promoter activity to inducer IPTG using five versions of RBS harboured by E. coli BL21*(DE3) in Luria broth media at 30āĀ°C. (c) Optimization of fatty acid production by balancing the RBS strength of GLY and FAS modules in mGLY-lACA-hFAS construct. GLY and FAS modules were expressed either under the control of a weak RBS (RBS32, relative strength 1), moderate RBS (RBS29, relative strength 2) or strong RBS (wild type, relative strength 3). All experiments were performed in triplicate and error bars show s.d. with 95% CI.
