Figure 4: Fed-batch cultivation of engineered strain in a 20-l bioreactor.
From: Modular optimization of multi-gene pathways for fatty acids production in E. coli
(a) Time course of dissolved oxygen (DO), pH and the total glucose (in g) fed in the fermenter. Forty percentage glucose (w/w) was fed at a constant rate of 1.235āmlāmin-1. pH was maintained at 6.8 by a proportional-integral derivative (PID) controller by feeding 5āN ammonium hydroxide. DO was maintained by sparging the fermenter with air or pure oxygen. (b) Time course profile of cell growth (OD), net residual glucose and total fatty acid production of the strain BL21ĪfadD expressing pCDM4-[RBS29]-GLY, pACM4-ACA and pETM6-FAS in 20-l bioreactor. Net residual glucose was calculated based on the difference between the total amount of glucose fed and the instantaneous glucose left. Fermentation assays were performed in duplicate and error bars show s.d. with 95% CI.
