Figure 1: Pile-up diagram of cereal pullulanase peptide sequences of the N-terminal domain region.
From: Allelic variation at a single gene increases food value in a drought-tolerant staple cereal
Black arrows highlight variant amino-acid positions between sorghum pullulanase allele types. Amino-acid tracks are given with pI tracks below; pI track bars are proportional to residue charge (positive is tall and blue; negative is low and black). From top to bottom the sequence tracks are: graphical representation of level of consensus identity, SbPUL-GD region with four amino acids found to differ from Sb06g001540.1 gene model (red underlay labelled 1) and the two polymorphic residues responsible for the GD to RA transition (red underlay labelled 2 and 3), SbPUL-RA, Zpu1 from Zea mays (NP_001104920), HvLD from H. vulgare (after Vester-Christensen et al.25), and OsPUL from Oryza sativa (CAE02111.2).
