Figure 5: Microglial activation is significantly increased in high-dose smoke-treated animals.
From: Smoking exacerbates amyloid pathology in a mouse model of Alzheimer’s disease
Light microscopic images of Iba1-immunoreactivity (specific for microglial cells) shown in cortex (a) and hippocampus (b) of control, low-dose and high-dose cigarette smoke-treated mice. Higher magnification images of Iba1-staining show the increased number of hypertrophied activated microglial cells (arrows in d) that appear in the cortex of the high-dose-treated animals compared with the control group (c). Activated microglial cells were statistically increased in high-dose-treated mice in both cortical (e) and hippocampal (f) areas compared with low-dose and untreated age-matched mice. I–VI: cortical layers; cc: corpus callosum; so: stratum oriens; sp: stratum pyramidale; sr: stratum radiatum; slm: stratum lacunosum moleculare. Data (mean±s.e.m.) was analysed by one-way ANOVA followed by Tukey’s multiple comparisons test. n=5–6 animals/group, four sections per animal. Scale bar: 50 μm (a–b), 25 μm (c–d). *P<0.05.
