Figure 2: Disruption of peptidoglycan architecture visualized by AFM.
From: Cell wall elongation mode in Gram-negative bacteria is determined by peptidoglycan architecture
(a) Targets for lysozyme and ATL amidase. (b) Sacculus fragment from a sample broken by the French press imaged under water, showing persistence of banding outwith the geometrical constraints of cell shape (scale bar, 50 nm; height, 4 nm). (c) In situ lysozyme digestion of sacculus under buffer A (scale bar, 100 nm; height, 10 nm). Trapped cytoplasmic material is resistant to the enzyme and acted as a fiduciary marker (circles). (d) In situ ATL amidase digest of sacculus under buffer A (scale bar, 100 nm; height 10 nm).
