Figure 3: Peptidoglycan insertion visualized by fluorescent vancomycin labelling.
From: Cell wall elongation mode in Gram-negative bacteria is determined by peptidoglycan architecture
(a) E. coli (MG1655) cells showing septal labelling, deconvolved cells showing apparently discontinuous labelling of the cylindrical part of the cell wall and sacculi, demonstrating specificity of labelling. (b) E. coli (MC1061) sacculi, similar to those of E. coli (MG1655). (c) E. coli (MG1655 ΔdacA) cells showing characteristic morphological defects compared with wild type, and increased level of labelling, deconvolved cells (showing apparently discontinuous labelling) and sacculi. (d) C. crescentus (NA1000) sacculi showing similar labelling pattern to E. coli. (f) STORM images of E. coli (MG1655) sacculi showing foci of insertion. (e) STORM images of C. crescentus (NA1000) sacculi showing a similar pattern of foci with E. coli (MG1655); (a–d) Scale bars, 5 μm; (e,f) scale bars, 1 μm.
