Figure 3: Shear stress frequency harmonics contribute to inflammatory phenotype.

(a,b) Frequency harmonic content was swapped between control waveforms using Fast Fourier transforms (FFT) to create new ‘mutated’ waveforms for harmonics 1–8. (c,d) The effect of mutating frequency harmonics on NF-κB activity was assessed, along with proinflammatory gene E-Selectin and anti-inflammatory gene, KLF2. Statistics: *P<0.05 for ANOVA (c,d), N=4–7.