Figure 7: Oxidative stress inhibits USP1 in complex with UAF1.
From: Reversible inactivation of deubiquitinases by reactive oxygen species in vitro and in cells
(a) The purified USP1–UAF1 complex. (b) The activity of the USP1–UAF1 complex is inhibited by H2O2. Where indicated, the USP1–UAF1 complex was treated with H2O2 (0.5 mM, 5 min). The DUB activities were determined by the Ub-AFC assay. (c) USP1 inhibition by H2O2 is reversed by DTT. The activity of the H2O2-treated USP1–UAF1 complex was measured. Where indicated, DTT was added to the reaction. (d) A model depicting the redox regulation of DUBs.
