Figure 1: Bone marrow-derived cells home to the PVN after fasting.
From: Haematopoietic cells produce BDNF and regulate appetite upon migration to the hypothalamus
(a) Cerebrum of wild-type mice receiving BMT from GFP-TG mice. Scale bar in lower right panel, 50 μm. LH, lateral hypothalamus; SON, supraoptic nucleus; 3 V, third ventricle. (b) GFP-positive cells in the PVN (delineated by dotted lines) of fed (left) or fasted (16 h, right) mice. Scale bars, 50 μm. (c) Percent of GFP-positive cells in the PVN following fasting (n=3–9). (d) Comparison of GFP-positive cells in other hypothalamic nuclei in fed and fasted mice (n=3–7). DMH, dorsomedial hypothalamus; VMH, ventromedial hypothalamus; ARC, arcuate nucleus. (e) Co-localization of various markers with the GFP-positive cells. Iba1, ionized calcium-binding adaptor molecule 1; RCA-1, ricinus communis agglutinin-1; Mac-1, macrophage antigen complex 1; GFAP, glial fibrillary acidic protein. (f) Percentage of cells positive for both GFP and the indicated markers in the PVN of fed or fasting (16 h) mice (n=4). Scale bars, 10 μm. All data represent the means±s.d. *P<0.05.
