Figure 1: Nitric oxide negatively regulates the cytokinin response.
From: S-nitrosylation of phosphotransfer proteins represses cytokinin signaling
(a,b) Analysis of the relative length of the primary roots (a) and hypocotyls (b) of seedlings treated with or without BA. The lengths of roots (a) or hypocotyls (b) not exposed to cytokinin treatment are set at 100% (n≥30) in each genotype. (c) Analysis of the root meristem cell number of seedlings treated with or without BA (n=20). (d) Shoot regeneration capacity of the hypocotyl explants cultured for four weeks in the presence of various concentrations of 2-isopentenyladenine (iPA). (e) Analysis of ARR5, ARR6 and ARR7 expression by qRT–PCR in 10-day-old seedlings treated with 5 μM BA. (f) Confocal microscopy of TCS-GFP reporter activity in Columbia-0 (Col-0) and gsnor1-3 roots. (g) TCS-GFP reporter activity in the roots of 7-day-old Col-0 seedlings grown in the absence (control) or presence of GSNO (400 μM) or sodium nitroprusside (SNP) (100 μM). Error bars indicate s.d.; * and ** indicate P<0.05 and P<0.01, respectively, (Student’s t-test).
