Figure 5: Reduced cytokinin response by a nitrosomimetic mutation.
From: S-nitrosylation of phosphotransfer proteins represses cytokinin signaling
(a,b) Analysis of the relative length of roots (a) and hypocotyls (b) in response to the cytokinin BA (numbers refer to the transgenic lines; n≥30). (c) Analysis of the shoot regeneration capacity of hypocotyl explants cultured for 2 weeks in the presence of various concentrations of 2-isopentenyladenine (iPA). (d) Analysis of root xylem development. Protoxylem and metaxylem are indicated by yellow and white arrows, respectively. ‘Cont’ indicates the respective mutants without a transgene in (a–c). All data are presented as mean values of three independent experiments. Error bars indicate s.d.; **indicates P<0.01 (Student’s t-test).
