Figure 4: OsMADS57 inhibits D14 expression.
From: The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14
(a) D14 promoter sketch showing CArG-boxes. (b) EMSA of OsMADS57 binding the cis-elements. The amount of protein loaded in each lane was equal. (c) ChIP assay of 2-week-seedling to show OsMADS57-binding regions in D14 promoter. Regions I and II contain the probes used in EMSA. The UBIQUITIN (Ub) promoter was used as a control. (d) Yeast one-hybrid analysis. The effectors contained the GAL4-activation domain. (e) Transcriptional repression activity assay in Arabidopsis protoplasts. S1Δ and/or S2Δ, deletion of site 1 and/or 2. (f) D14 expression in roots (R), culms (C), sheaths (Sh), leaves (L), panicles (P) and axillary buds(Ab). (g) RNA in situ assay for D14. Scale bars, 100 μm.
