Figure 4: Q₅₆-YFP levels increase and its molecular state changes when Hsp104 or Pho5 are lost.

(a) Western blotting against GFP reveals that the expression of the Q₅₆-YFP protein is not diminished in any of the Group I knockout strains. Instead, expression is increased in the GdnHCl-treated WT MAT a strain and the strains with deletions of HSP104 and PHO5. Hsp82 was used as a loading control. (b) Native agarose gel electrophoresis reveals oligomeric forms of Q₅₆-YFP upon the addition of GdnHCl and the deletion of HSP104 or PHO5. This is not the case when other genes of Group I are deleted. In both panels, the lysates from WT MAT a transformed with pQ0 and pQ30 were diluted 15-fold to enable simultaneous detection of all lysates on the same gel and western blot.