Figure 5: pQ56 interferes with specific cellular structures.

Fluorescent reporter strains were transformed with cytostatic and non-cytostatic polyQ constructs and the effect on reporter structures was examined. The arrowheads point to cells representative for a certain localization pattern and coloured according to their class for which the averaged quantiation result from three independent biological replicates is visualized in the pie chart to the right of each micrograph (Supplementary Fig. S13 for corresponding bar charts, indicating error margins). The scale bar represents 5 μm in all panels. (a) Tub1-GFP is a marker for the spindle and only minor changes in the amount of cells with assembled tubulin structures can be detected. However, elongated spindles are slightly more frequent in pQ56cherry transformants and never extend into a bud or daughter. (b) Spindle pole bodies, labelled with Nud1-GFP may exhibit no, a single focal, or two focal depositions. They seem unaffected by pQ56cherry (c) Cdc10-GFP localizes to the septin ring, but also may be found not localized to a specific structure or exhibit granular or focal appearance to a low extent. When pQ56cherry is present, ring association is found less frequently, being substituted by an increased amount of granular or multifocal localization. (d) The effect observed for Cdc10-GFP is even more pronounced for the septin Shs1. Upon transformation with pQ56cherry ring association is substituted by a majority of cells exhibiting multifocal deposition of the septin.