Figure 1: HPLC fractions derived from urine are tested for the MHC model peptide SIINFEKL by ELISA. | Nature Communications

Figure 1: HPLC fractions derived from urine are tested for the MHC model peptide SIINFEKL by ELISA.

From: Mouse urinary peptides provide a molecular basis for genotype discrimination by nasal sensory neurons

Figure 1

(a) A fraction of urine from a B6/OVA+ mouse gives rise to a strong signal, while the corresponding preparations from a B6/OVA+/β2m−/− mouse and B6 mice are negative. Spiked synthetic SIINFEKL elutes at the same retention time as the substance detected in B6/OVA+ urine. (b) SIINFEKL concentrations measured in the urine of individual B6/OVA+ mice (n=9); there was no obvious correlation with the age of the mice (Supplementary Fig. S6). For comparison, results for individual B6/OVA+/β2m−/− (n=4) and B6 wild type (n=5) mice are shown. The two data points printed in red are derived from two separate urine samples of the same mouse. A two-tailed, heteroskedastic Student's t-test was applied with Bonferroni correction for two comparisons.

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