Table 1 Urinary peptides with MHC class I binding motif.

H2 allele	Peptide	Origin of peptide	Location of protein ⁶⁰
K^b	NKQEFGWI	Carboxylesterase 1B, 1C, 1D and/or 1E	e, i
	*LNSVFDQL	Kidney androgen-regulated protein	e
	LAPQPFLRV	Leucine-rich alpha-2-glycoprotein	e, mem (?)
D^b	FNIQNREPLI	ATP-binding cassette, sub-family A, member 13	mem
	KELQNSIIDL	Kidney androgen-regulated protein	e
	*TRVLNLGPI	Uromodulin (Tamm-Horsfall urinary glycoprotein)	e, mem
K^d	*VYRPDQVSIL	Deoxyribonuclease-1	e, i
	LYWVDVERQV	Pro-epidermal growth factor	mem
	LFKDSAFGL	Serotransferrin	e
L^d	YSMPPIVRF	Alpha-1-antitrypsin 1–3 and/or 1–4	e
	*PAVRGFSL	LDL-receptor-related protein 2 (Megalin)	mem
	SSDIKERF	Major urinary protein (No. 1–8, 11 and/or 12)	e
	LSSDIKERF	Major urinary protein (No. 1–8, 11 and/or 12)	e
	PSFVPLSKF	Napsin-A (Kidney-derived aspartic protease-like protein)	Lysosomal
	*PVESKIYF	Pro-epidermal growth factor	mem
	LSRQMGMVF	Pro-epidermal growth factor	mem
	GPVQGTIHF	Superoxide dismutase [Cu-Zn]	i, e

e, extracellular; i, intracellular; mem, plasma membrane.
Urine of five mouse strains (B10, B10.D2, B6, B6/β₂m^−/− and BALB/c) was collected in metabolic cages overnight. For each strain, urine mixtures of 4 to 5 mice were subjected to MS analysis. The MHC anchor amino acids are marked in bold. Asterisks indicate peptides that were chosen for quantification (see Table 2 for two examples).

Quick links

Search