Table 2 Urinary concentrations of two peptides with MHC class I binding motif and their extended versions.

From: Mouse urinary peptides provide a molecular basis for genotype discrimination by nasal sensory neurons

  

Urinary concentration in 10 −8  M

 

Mouse strain

B10

B10.D2

B6

B6/β 2 m −/−

BALB/c

Peptide

MHC allele

b

d

b

(b)

d

LNSVFDQL

H2-Kb

97

100

44

68

35

LNSVFDQLGSY

—

99

154

212

265

194

Ratio [LNSVFDQL]/[LNSVFDQLGSY]

 

0.98

0.65

0.21

0.26

0.18

TRVLNLGPI

H2-Db

4.1

3.0

0.9

1.4

4.1

IDQTRVLNLGPI

—

11.9

15.6

14.4

20.0

14.1

Ratio [TRVLNLGPI]/[IDQTRVLNLGPI]

 

0.35

0.19

0.06

0.07

0.29

  1. The MS-based quantification was done with the help of the corresponding synthetic 13C515N1-valine-containing peptides spiked into manually collected urine of five mouse strains. Ratios for urinary concentrations of the MHC motif peptides and their extended forms were calculated in order to correct for possible strain-specific differences in protein expression. Note that B6/β2m−/− mice do not express functional MHC class I molecules due to the lack of the β2m subunit.
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